Hsp90 β is critical for the infection of severe fever with thrombocytopenia syndrome virus

Bo Wang; Leike Zhang; Fei Deng; Zhihong Hu; Manli Wang; Jia Liu

doi:10.1016/j.virs.2023.11.008

February 2024

Citation: Bo Wang, Leike Zhang, Fei Deng, Zhihong Hu, Manli Wang, Jia Liu. Hsp90 β is critical for the infection of severe fever with thrombocytopenia syndrome virus .VIROLOGICA SINICA, 2024, 39(1) : 113-122. http://dx.doi.org/10.1016/j.virs.2023.11.008

Hsp90 β is critical for the infection of severe fever with thrombocytopenia syndrome virus

Bo Wang ^a,b ,
Leike Zhang ^a ,
Fei Deng ^a ,
Zhihong Hu ^a ,
Manli Wang ^a ,
Jia Liu ^{a
,,}

a. State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China;
b. The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, 511436, China

Corresponding author: Jia Liu, liujia@wh.iov.cn
Received Date: 21 February 2023
Accepted Date: 22 November 2023
Available online: 24 November 2023

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV) is an emerging disease in East Asia with a fatality rate of up to 30%. However, the viral-host interaction of SFTSV remains largely unknown. The heat-shock protein 90 (Hsp90) family consists of highly conserved chaperones that fold and remodel proteins and has a broad impact on the infection of many viruses. Here, we showed that Hsp90 is an important host factor involved in SFTSV infection. Hsp90 inhibitors significantly reduced SFTSV replication, viral protein expression, and the formation of inclusion bodies consisting of nonstructural proteins (NSs). Among viral proteins, NSs appeared to be the most reduced when Hsp90 inhibitors were used, and further analysis showed that their translation was affected. Co-immunoprecipitation of NSs with four isomers of Hsp90 showed that Hsp90 β specifically interacted with them. Knockdown of Hsp90 β expression also inhibited replication of SFTSV. These results suggest that Hsp90 β plays a critical role during SFTSV infection and could be a potential target for the development of drugs against SFTS.
- Severe fever with thrombocytopenia syndrome virus (SFTSV)
- , Heat-shock protein 90
- , Hsp90 β
- , Host-virus interaction
- , Nonstructural protein

Electronic Supplementary Material

10.1016j.virs.2023.11.008-ESM.docx
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